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KMID : 0359320030430030429
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Korean Journal of Veterinary Research
2003 Volume.43 No. 3 p.429 ~ p.433
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Determination of Ceftiofur in Bovine Serum by Liquid Chromatography-Electrospray Mass Spectrometry
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Lim, Jong-hwan/임종환
Jang, Beon-su/Park, Byung-kwon/Yun, Hyo-in/장범수/박병권/윤효인
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Abstract
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This study was aimed to develop a more rapid, simple and sensitive method to determine ceftiofur in bovine serum using LC/MS with electrospray interface. Separation was achieved on the NovaPak C_(18) reverse phase column. The mobile phase consisted of 0.1％ acetic acid in water (A) and acetonitrile (B) and gradiently flowed at the rate of 0.4 mL/min. As a result of analysis of blank muscle samples, matrix interference was not shown. Limit of detection and limit of quantitaion was 5 ng/g and 20 ng/g, respectively. The values of precision and recovery satisfied the guideline of NVRQS. The precision and recovery developed in this method are suitable and sensitive to determine the concentration of ceftiofur in the bovine serum. These results could be applied for the confirmation and quantification in the biofluid.
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KEYWORD
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